Journal: Oncology Reports

Article Title: Heparin-binding epidermal growth factor-like growth factor is a potent regulator of invasion activity in oral squamous cell carcinoma

doi: 10.3892/or.2011.1616

Figure Lengend Snippet: Expression levels of EGF family mRNA in HSC3 cells. Amphiregulin (AREG), epigen (EPHN), epiregulin (EREG), HB-EGF (HBEGF), TGF-α (TGF), betacellulin (BTC), EGF and β-actin mRNA expression levels in HSC3 cells were analyzed by RT-qPCR.

Article Snippet: HSC3 cells were transfected with HB-EGF siRNA oligos (sense 5′-GGACCCAUGUCUUCGGAAA-3′ and antisense 5′-UUUCCGAAGACAUGGGUCC-3′) or control scrambled siRNA oligos (sense 5′-AUCCGCGCGAU AGUACGUA-3′ and antisense 5′-UACGUAGUAUCGCGCG GAU-3′) (B-Bridge International, Mountain View, CA) using Lipofectamine™ RNAiMAX (Invitrogen) according to the manufacturer’s instructions, followed by incubation for 48 h at 37°C in a humidified atmosphere of 5% CO 2 in air.

Techniques: Expressing, Quantitative RT-PCR

Journal: Oncology Reports

Article Title: Heparin-binding epidermal growth factor-like growth factor is a potent regulator of invasion activity in oral squamous cell carcinoma

doi: 10.3892/or.2011.1616

Figure Lengend Snippet: Knockdown of heparin-binding epidermal growth factor-like growth factor (HB-EGF) affects invasion activity in HSC3 cells. (A) Expression of HB-EGF mRNA in HSC3 cells treated with siRNAs was determined by real-time RT-PCR. The amount of HB-EGF mRNA was significantly reduced in HB-EGF siRNA-treated HSC3 cells compared with that in control no-siRNA or scrambled-siRNA-treated HSC3 cells. (B) Effect of HB-EGF on HSC3 cell proliferation. The MTT assay showed similar proliferation rates among cells treated with HB-EGF siRNA (closed circle), no-siRNA (triangle), and scrambled-siRNA (open circle). (C) Invasion activity of HB-EGF siRNA-transfected HSC3 cells as determined by the Matrigel invasion assay. The invasion index represents the ratio of cells migrating through a Matrigel-coated membrane/cells migrating through a control non-coated membrane. No-siRNA and scrambled-siRNA transfectants were highly invasive, whereas the invasiveness of HB-EGF siRNA-transfected HSC3 cells was significantly lower.

Article Snippet: HSC3 cells were transfected with HB-EGF siRNA oligos (sense 5′-GGACCCAUGUCUUCGGAAA-3′ and antisense 5′-UUUCCGAAGACAUGGGUCC-3′) or control scrambled siRNA oligos (sense 5′-AUCCGCGCGAU AGUACGUA-3′ and antisense 5′-UACGUAGUAUCGCGCG GAU-3′) (B-Bridge International, Mountain View, CA) using Lipofectamine™ RNAiMAX (Invitrogen) according to the manufacturer’s instructions, followed by incubation for 48 h at 37°C in a humidified atmosphere of 5% CO 2 in air.

Techniques: Binding Assay, Activity Assay, Expressing, Quantitative RT-PCR, MTT Assay, Transfection, Invasion Assay

Journal: Oncology Reports

Article Title: Heparin-binding epidermal growth factor-like growth factor is a potent regulator of invasion activity in oral squamous cell carcinoma

doi: 10.3892/or.2011.1616

Figure Lengend Snippet: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) upregulates MMP-9 expression. (A) MMP-9 mRNA expression and collagenase activity in siRNA-treated HSC3 cells was determined by RT-PCR and zymography, respectively. The MMP-9 mRNA level was reduced in HB-EGF siRNA-treated cells compared with the levels in control no-siRNA and scrambled-siRNA-treated HSC3 cells. (B) The addition of s-HB-EGF to the culture medium upregulated MMP-9 mRNA levels in HSC3 cells.

Article Snippet: HSC3 cells were transfected with HB-EGF siRNA oligos (sense 5′-GGACCCAUGUCUUCGGAAA-3′ and antisense 5′-UUUCCGAAGACAUGGGUCC-3′) or control scrambled siRNA oligos (sense 5′-AUCCGCGCGAU AGUACGUA-3′ and antisense 5′-UACGUAGUAUCGCGCG GAU-3′) (B-Bridge International, Mountain View, CA) using Lipofectamine™ RNAiMAX (Invitrogen) according to the manufacturer’s instructions, followed by incubation for 48 h at 37°C in a humidified atmosphere of 5% CO 2 in air.

Techniques: Binding Assay, Expressing, Activity Assay, Reverse Transcription Polymerase Chain Reaction, Zymography

Journal: Oncology Reports

Article Title: Heparin-binding epidermal growth factor-like growth factor is a potent regulator of invasion activity in oral squamous cell carcinoma

doi: 10.3892/or.2011.1616

Figure Lengend Snippet: Shedding of HB-EGF upregulates MMP-9 expression through EGFR receptor activation. (A) The TACE inhibitor TAPI-2 reduced MMP-9 mRNA levels in HSC3 cells. (B) The EGFR inhibitor AG1478 downregulated MMP-9 mRNA levels in HSC3 cells

Article Snippet: HSC3 cells were transfected with HB-EGF siRNA oligos (sense 5′-GGACCCAUGUCUUCGGAAA-3′ and antisense 5′-UUUCCGAAGACAUGGGUCC-3′) or control scrambled siRNA oligos (sense 5′-AUCCGCGCGAU AGUACGUA-3′ and antisense 5′-UACGUAGUAUCGCGCG GAU-3′) (B-Bridge International, Mountain View, CA) using Lipofectamine™ RNAiMAX (Invitrogen) according to the manufacturer’s instructions, followed by incubation for 48 h at 37°C in a humidified atmosphere of 5% CO 2 in air.

Techniques: Expressing, Activation Assay

Journal: Molecular Medicine

Article Title: Transposition element MERVL regulates DNA demethylation through TET3 in oxidative-damaged mouse preimplantation embryos

doi: 10.1186/s10020-025-01143-3

Figure Lengend Snippet: DEGs associated with oxidative damage in zygotes. A Volcano plot showing DEGs in 2-cell embryos following oxidative damage to zygotes. B Functional analysis of DEGs. C DEGs enriched in the apoptotic pathway, with up-regulated genes depicted in red and down-regulated genes in green. D The impact of oxidative damage on the expression of TEs. E The impact of oxidative damage on MERVL expression in fertilized eggs. F Immunofluorescence analysis was used to analyze the impact of oxidative damage on MERVL expression. G Statistical results representing the expression of MERVL in different groups after oxidative damage in early embryos. Bar = 20 μm. Data are presented as means ± SDs, collected from three independent experiments, each involving more than five embryos. Differences among groups were analyzed using Student’s t-test. *P < 0.05 vs. control; **P < 0.01; ***P < 0.001. DEGs: differentially expressed genes

Article Snippet: The sequence of the antisense MERVL S-oligo is 5ʹ-GATTCATCCTTGTACTTCTG-3ʹ (GenePharma).

Techniques: Functional Assay, Expressing, Immunofluorescence, Control

Journal: Molecular Medicine

Article Title: Transposition element MERVL regulates DNA demethylation through TET3 in oxidative-damaged mouse preimplantation embryos

doi: 10.1186/s10020-025-01143-3

Figure Lengend Snippet: MERVL plays a crucial role in maintaining the genome stability of pre-implantation embryos. A Knockdown of MERVL using ASOs. B Electroporation of fluorescently labeled controls using a voltage of 800 V, pulse duration of 20 ms, repeated twice. C Representative images of immunofluorescence staining for MERVL-Gag protein, counterstained with DAPI, in control and MERVLKD embryos at the late two-cell stage, derived from three independent experiments. Scale bars, 20 µm. D Representative phase-contrast images of 4.5 dpc blastocysts in control and MERVL-KD embryos, from three independent experiments. Scale bars, 100 µm. E Representative images of immunofluorescence staining for TUNEL apoptosis in control and MERVL-KD embryos at the blastocyst stage, from three independent experiments. Scale bars, 20 µm. F γH2AX fluorescence intensity in control and MERVL-KD embryos. Data are presented as means ± SDs. *P < 0.05 vs. control; **P < 0.01; ***P < 0.001. ASO: antisense oligonucleotide; KD: knockdown; γH2AX: gamma H2A histone family member X; dpc: days post-coitum

Article Snippet: The sequence of the antisense MERVL S-oligo is 5ʹ-GATTCATCCTTGTACTTCTG-3ʹ (GenePharma).

Techniques: Knockdown, Electroporation, Labeling, Immunofluorescence, Staining, Control, Derivative Assay, TUNEL Assay, Fluorescence

Journal: Molecular Medicine

Article Title: Transposition element MERVL regulates DNA demethylation through TET3 in oxidative-damaged mouse preimplantation embryos

doi: 10.1186/s10020-025-01143-3

Figure Lengend Snippet: MERVL regulates DNA methylation changes in early embryos. A The expression of MERVL is unaffected by DNA methylation levels. B Expression of DNA methylation enzymes in two-cell embryos following MERVL-KD. C Expression of DNA methylation enzymes in blastocysts after MERVL-KD. D Establishment of the MERVL-KD and rescue groups. E DNA methylation levels in two-cell embryos from the MERVL-KD and rescue groups. F DNA methylation levels in blastocysts from the MERVL-KD and rescue groups. G Expression levels of DNMT1, TET3 in the MERVL-KD and rescue groups. MERVL: murine endogenous retrovirus-like; MERVL-KD: MERVL knockdown; DNMT1: DNA methyltransferase 1; TET3: ten-eleven translocation 3

Article Snippet: The sequence of the antisense MERVL S-oligo is 5ʹ-GATTCATCCTTGTACTTCTG-3ʹ (GenePharma).

Techniques: DNA Methylation Assay, Expressing, Knockdown, Translocation Assay